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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) <t>Energy-dispersive</t> <t>X-ray</t> spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) <t>XRD</t> analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) Energy-dispersive X-ray spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) XRD analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

Journal: Bioactive Materials

Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

doi: 10.1016/j.bioactmat.2026.02.043

Figure Lengend Snippet: Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) Energy-dispersive X-ray spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) XRD analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

Article Snippet: X-ray diffraction (XRD) patterns were conducted on a Bruker D8 ADVANCE X-ray diffractometer using Cu-Kα radiation (λ = 1.5418 Å).

Techniques: Spectroscopy, Concentration Assay